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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 2: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 3: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Topic 4: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 5: Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
| Topic 6: Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
| Topic 7: Blood Banking | 15–20% | - Transfusion practice and testing - Blood group systems and immunology - Blood products and components |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
Which of the following fields is NOT an aspect of clinical microbiology?
A. bacteriology
B. pathology
C. parasitology
D. virology
Question 2
Two CSF specimens were sent to the Lab with the following results:
Tube #1 = 11,200 rbc/µL
Tube #2 = 300 rbc/µL
The results on these CSF specimens are indicative of:
A. an old intracranial bleeding episode faulty lab equipment
B. a recent subarachnoid hemorrhage
C. an infection
D. a traumatic tap
Question 3
The indole (modified Kovacs' reagent) is used to assess the ability of certain bacteria to produce indole by the deamination of tryptophan. Indole reacts with aldehyde in the Kovac's reagent to produce a red color. An alcoholic layer holds the red color as a ring at the top of the tube used for the idole test.
If a bacteria produces the enzyme tryptophanase to break down the amino acid tryptophan, which of the following tests will be positive with Kovac's reagent?
A. citrate test
B. esculin test
C. oxidase test
D. indole test
E. catalase test
Question 4
Calculation:
Cells Counted (in this case the average of both sides) X dilution factor (in this case 100) / # of squares counted (in this case 9) X area of each square (1mm2) X 0.1mm (depth factor) So, in this problem:
158 x 100 / 9 x 1 x 0.1mm = 17555.55/mm3 (can be converted to 17.5 x 109/L*)
*There are 1,000,000 mm3 in a liter (L). So 17555.55 X 1,000,000 = 17.5 x 109/L A manual white blood cell count was performed by the hematology technologist. The cell counts for both sides were 152 and 164 respectively. All nine large squares were counted on each side. The dilution for this kit was pre-measured at 1:100. What should the technologist report as the white cell count?
A. 177.5 x 10^9/L
B. 175 x 10^9/L
C. 17.5 x 10^9/L
D. 1.75 x 10^9/L
Question 5
Certain recipients have increased risk for developing TA-GVHD. They are:
Neonates less than 4 months of age
Fetuses
Recipients with a congenital or acquired immunodeficiency, such as bone marrow or stem cell recipients, and patients receiving chemotherapy recipients of donor units from a blood relative Which of the following patients are at risk for transfusion-associated graft versus host disease (TA-GVHD) and require irradiated cellular blood products? (Choose all that apply)
A. Neonates less than 4 months of age
B. Recipients of donor units known to be from a blood relative.
C. Patients with a history of allergic reactions.
D. Patient receiving chemotherapy who are immunocompromised.
E. Patients with chronic anemias.
Solutions:
| Question 1 Answer: B | Question 2 Answer: D | Question 3 Answer: D | Question 4 Answer: C | Question 5 Answer: A,B,D |

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